Solute–Solvent Interactions in Hydrophilic Interaction Liquid Chromatography: Characterization of the Retention in a Silica Column by the Abraham Linear Free Energy Relationship Model

Abstract The Abraham linear free energy relationship model has been used to characterize a hydrophilic interaction liquid chromatography (HILIC) silica column with acetonitrile/water and methanol/water mobile phases. Analysis by the for phases points solute volume hydrogen bond basicity as main properties affecting retention, whereas acidity, dipolarity polarizability practically do not affect it. Formation of cavity is easier in acetonitrile-rich than aqueous stationary phase, hence increase decreases retention. Conversely, acidity stronger phase thus an increases Results are similar difference that significant too. Increase retention showing methanol must be better acceptors acetonitrile ones, even water-rich results like ones obtained zwitterionic HILIC columns bonded or polymer supports phases, but different indicating bonding support plays important role Comparison RPLC characterized systems confirms complementarity because same reversed coefficients. least retained solutes most HILIC.